Mass production of Beauveria bassiana (white muscardine fungus)

Materials required

• Sorghum
• Water
• Chalk powder
• Autoclave

Methodology

Soak 1 Kg of Sorghum in water for 48 hours. Replace water after 24 hrs, after 48 hrs. rinse water completely. Separate equally in 10-15 flasks and plug with hard cotton cushion and wrap with double aluminum foil. Sterilize for 40 minutes with 21 psi. Inoculate the each flask containing jowar with 2-3 drop of nucleus culture after cooling. Beauveria culture will grow fully after 20-25 days. Mix 2 Kg of chalk powder in Beauveria culture and dry in shade.

Dose

1 gram/liter of water or 1 Kg/1000 liter of water/ha (Repeat application after 10-20 days interval)

Source: Skill Development of Farmers for Production of IPM inputs in IPM Seva Kendra